Bioinformatics Introduction

Objectives

  1. What is Bioinformatics.
  2. What is the general workflow of bioinformatics.
  3. What kind of knowledge you need to know to better understand bioinformatics.

What is Bioinformatics

  • Bioinformatics is a field that uses computational tools and statistical methods to help biologists organize, analyze, and interpret biological data.
  • It helps researchers better understand their data and draw biological conclusions from the results.

What is the general workflow of bioinformatics.

3 steps: Get data → Process data → Interpret data

Overal general bioinformatics workflow: Biological question → Sample collection → Data generation → Quality control → Data preprocessing → Mapping / assembly / quantification → Downstream analysis → Visualization → Biological interpretation

What kind of knowledge you need to know to better understand bioinformatics.

Need to know basic biology concepts

  1. What is ORF?
    • ORF is Open Reading Frame

Need to know different kinds of sequencing techniques (Molecular Biology)

  1. What is high throughput sequencing
  2. What is RNA-seq

Need to know different tools to work with data and common data file

  1. What is FASTQ file and how to read it
  2. What is FASTQC tool and what is it did
  3. Strategies for analyzing genome resequencing
  4. Strategies for analyzing RNASeq
  5. Strategies for analyzingmicrobiome sequencing data
  6. Strategies for analyzing high throughput biological data
  7. What is BioPython
  8. What is BLAST
    • Blast is Basic Local Alignment Search Tool