W2K Questions 1. What is FASTQ format 2. Know what is Quality Scores 3. Signal decay and phasing 4. What is adapter
FASTQ format:
| Line | Description |
|---|---|
| 1 | Always begins with ‘@’ and then information about the read |
| 2 | The actual DNA sequence |
| 3 | Always begins with a ‘+’ and sometimes the same info in line 1 |
| 4 | Has a string of characters which represent the quality scores; must have same number of characters as line 2 |
Steps:
- FASTQE visualize fastq files with emoji’s 🧬😎 (Galaxy Version 0.3.1+galaxy0)
- This tool can convert
- FastQC
- Cutadapt
Short read session
Long read session
nanoplot